The human 8-oxoguanine DNA glycosylase and xeroderma pigmentosum group haplotypes variation in x- ray employees

Abstract

Author(s): Moayad Aziz Abdulqadir*, Hussain Ghazil, Mariam Alaa Toama, Liwaa Hussein Mahdi, Jamal A. Aljabbar Attawi, Khulood Majid Alsaraf and Zahraa F. Hassan

DNA repair genes polymorphism including The human 8-oxoguanine DNA glycosylase (hOGG ) Ser326Cys and Xeroderma Pigmentosum Group (XPD) Arg399Gln genes in the x-ray employees were studied in present study. PCR-SSCP technique was used to detect haplotype polymorphisms. The results found that the haplotypes distribution of both genes were significant association with cases, XPD have high percentage of di haplotype than control group in significant association (p=0.0019).The hOGG haplotype distribution in study groups shows significant association of di haplotype with cases than control group (P=0.000), both genes haplotypes were distributed according work time in cases per day to more and less than 6 hours, XPD showed non-significant association XPD (p=0.9395), uni and di haplotype more frequent in cases exposure to x-ray less than 6 hours per day. The hOGG appeared in significant association with work time (p=0.000) that more frequent of di haplotype in less than 6 hours category, di haplotype more frequent in case work less than 6 hours per day. The di haplotypes more frequent in worker with less than 6 hours per day in non-significant association (p=0.4132). The exposure day to x-ray classified to less and more than 4 days per week, XPD non-significant association with exposure day (p=0.798) about 50% of worker have uni haplotype that work less than 4 days. The hOGG haplotypes also non-significant association with exposure day (p=0.798), Different work periods were reported and classified to less and more than 10 years, XPD showed non-significant association (p=0.582) with work period, uni haplotype was frequent in same percentage in both categories. The hOGG also non-significant correlations with work periods (p=0.1775), di haplotype more frequent in worker with less than 10 years, the current results concluded that there were vital role of x-ray in the hOGG Ser326Cys and (Arg399Gln) XPD genes.

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