Micro RNA-145, -155 and -328 as biomarkers in breast cancer Iraqi women patients

Abstract

Author(s): Aseel S Mahmood*, Shahlaa M Salih, Mahmood A Muhi

Breast Cancer (BC) is the major issue in current therapy due to lack of sensitive and specific biomarker of tumor. It has been suggested by a number of studies that several miRNAs (miRNAs or miR) control human BC. Non-coding RNA molecules like miRNAs pave the way for several potential avenues of early cancer detection. Cancer risk has been associated with certain miRNA expression profiles. The goal of this study is to characterize the miRNA blood profiles of healthy controls and women in Iraq who have been diagnosed with breast cancer. Blood samples from 40 Breast Cancer (BC) patients and 40 healthy controls were analyzed utilizing real-time quantitative Polymerase Chain Reaction (qPCR) and the delta technique to determine the expression of 3 microRNAs (micro-328, micro-155, and micro-145). Micro-155 was found to be overexpressed, whereas micro-145 was shown to be down-regulated in patients. Expression of micro-328 has not altered much, as demonstrated by the fold change. Based on the findings, micro-155 and micro-145 expression levels have the potential to be used as important predictive indicators for BC patients and as interested biomarkers for future therapeutic interventions.

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