Detection of methylated gene (p16) in patients with head and neck squamous cell carcinoma using conventional PCR


Author(s): Ehab T Yassen and Mohammed Q Mohammed*

Introduction: The role of detecting abnormal methylations in the genes and other epigenetic abnormalities in the patients with HNSCC, like p16 gene in the DNA of human cells could guide us to detect carcinoma early.

Aim: To detection abnormal methylation in p16 gene in HNSCC.

Patients and Methods: This is a case-control and cross sectional combine study conducted in the Otolaryngology Department at Al-Yarmouk Teaching Hospital. Forty one patients enrolled, they were divided into two groups. Group-A included 21 patients have HNSCC and group-B included 20 patients as controls. Saliva was collected from oral cavity. PCR was performed to identify the DNA. This is subjected to methylations looking for the presence or absence of gene methylation.

Results: 21 patients in group-A, 16 were males, and 5 were females. 7 patients were positive for methylation in EXON-1, 5 were smokers and 2 were alcoholic, whereas 12 were positive for methylation in EXON-2, 8 were smokers and 2 were alcoholic. In group B: 7 patients were positive for methylation in EXON-1, 6 were smokers and 3 were alcoholic, whereas 4 were positive for methylation in EXON-2, all were smokers and 1 was alcoholic.

Conclusion: Hyper-methylation in specific EXON of gene p16 is a useful method for the detection of abnormal epigenetic which could lead to the development of HNSCC in high risk people. The detection of methylation in p16 correlates well with nodal involvement. EXON-2 methylation of p16 gene was significantly detected more than EXON-1 and more with HNSCC.

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    Submitted PhD thesis in Biotechnology at GITAM University, Vizag.

  • Yousef Alomi

    Yousef Alomi
    The Past Head, General Administration of Pharmaceutical Care at Ministry of Health,
    Saudi Arabia Critical Care/TPN
    Clinical Pharmacist Ministry of Health,
    Riyadh, Saudi Arabia.

  • Osamu Tanaka

    Osamu Tanaka
    Assistant Professor,
    Department of Radiation Oncology
    Asahi University Hospital
    Gifu city, Gifu, Japan

  • Maher Abdel Fattah Al-Shayeb

    Department of Surgical Sciences, Ajman University, UAE

  • Andrzej Zdziennicki

    Institute of Gynecology and Obstetrics, Medical University of Lodz, I Clinic of Gynecology and Gynecological Oncology (Lodz, Poland)

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