Effect of betel oil on of normal and cancer cells lines


Author(s): Zainab H.A. Al-Batat* and Ali A.A. Al-Ali

The present research concentrates on using both synthetic medications (metformin) and biological substances (betel oil) to use the MTT viability experiment to determine their toxicity against the cancer cell types AMGM-5, A549, HCAM, HCT-8, and HeLa as well as the normal cell type HBL100. The result shows decreased cell viability treated with betel oil and metformin. IC50 value of betel oil in A549, HCAM and HCT-8 was 12.5 µg/ml, 21.2 µg/ml, 12.6 µg/ml respectively, while the killing rates did not reach 50% for each of HBL-100, AMGM-5 and HeLa. Acridine orange/Ethidium bromide, Eosin and Hematoxylin after transaction betel oil and metformin were used to examine the structural and apoptotic changes in the cell lines. These changes included apoptosis and degradation as well as the appearance of necrotic cells.

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Awards Nomination

Editors List

  • Prof. Elhadi Miskeen

    Obstetrics and Gynaecology Faculty of Medicine, University of Bisha, Saudi Arabia

  • Ahmed Hussien Alshewered

    University of Basrah College of Medicine, Iraq

  • Sudhakar Tummala

    Department of Electronics and Communication Engineering SRM University – AP, Andhra Pradesh




  • Alphonse Laya

    Supervisor of Biochemistry Lab and PhD. students of Faculty of Science, Department of Chemistry and Department of Chemis


  • Fava Maria Giovanna


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