Effect of betel oil on of normal and cancer cells lines

Abstract

Author(s): Zainab H.A. Al-Batat* and Ali A.A. Al-Ali

The present research concentrates on using both synthetic medications (metformin) and biological substances (betel oil) to use the MTT viability experiment to determine their toxicity against the cancer cell types AMGM-5, A549, HCAM, HCT-8, and HeLa as well as the normal cell type HBL100. The result shows decreased cell viability treated with betel oil and metformin. IC50 value of betel oil in A549, HCAM and HCT-8 was 12.5 µg/ml, 21.2 µg/ml, 12.6 µg/ml respectively, while the killing rates did not reach 50% for each of HBL-100, AMGM-5 and HeLa. Acridine orange/Ethidium bromide, Eosin and Hematoxylin after transaction betel oil and metformin were used to examine the structural and apoptotic changes in the cell lines. These changes included apoptosis and degradation as well as the appearance of necrotic cells.

Share this article